Author : M.Nunes da Silva, M.E.Pintado, B.Sarmento, N.P.Stamford, M.W.Vasconcelos

Address : (aUniversidade Católica Portuguesa, CBQF – Centro de Biotecnologia e Química Fina – Laboratório Associado, Escola Superior de Biotecnologia, Rua Arquiteto Lobão Vital 172, 4200-374 Porto, PortugalbI3S-Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Rua Alfredo Allen 208, 4200-135 Porto, PortugalcCESPU, Instituto de Investigação e Formação Avançada em Ciências e Tecnologias da Saúde, Rua Central da Gandra 1317, 4585-116 Gandra, PortugaldPrograma de Pós-graduação em Ciências do Solo, Departamento de Agronomia, Universidade Federal Rural de Pernambuco, Av. Dom Manoel de Medeiros, s/n, Dois Irmãos, Recife, Pernambuco, Brazil)

Abstract : A biofertilizer with diazotrophic bacteria and a filamentous fungus increases Pinus pinastertolerance to the pinewood nematode (Bursaphelenchus xylophilus).

Journal : Biological Control

Volume No. : Volume 132

Publish Year : 2019

Page No. : 72-80

Main Data : The pine wilt disease (PWD), caused by the pine wood nematode (PWN) Bursaphelenchus xylophilus, is a devastating illness that mainly affects P. pinastertrees, and that poses great environmental and economic challenges. Current disease management involves the cut down of infected trees, tree fumigation, use of nematicides, or the control of the insect vector; however, these methodologies are expensive, labour-intensive and have limited success. The aim of this work was to evaluate the effect of a biofertilizer enriched with diazotrophic bacteria and a chitosan-producing fungus, Cunninghamella elegans, in inducing P. pinaster and P. pinea resistance against the PWN. In non-inoculated (control) P. pinaster plants, PWN population significantly increased (ca. 2.3-fold) throughout the experimental period, whereas in plants treated with 7.5 and 15% of biofertilizer nematode numbers were up to 36.3-fold lower than in control plants. In P. pinea, nematode numbers decreased with time for all biofertilizer concentrations tested, and P. pinea had up to 27.3-fold lower nematode counts than P. pinaster. In addition, the biofertilizer prevented the decrease of photosynthetic pigments and the reduction of water content in infected P. pinaster plants. In P. pinea the biosynthesis of phenolics increased in PWN-inoculated plants, especially in the presence of the biofertilizer. The addition of this biofertilizer to soils forested by P. pinastermay improve plant defence and could be a potentially simple and inexpensive strategy for the control of the PWD. Keywords: Biofertilizer; Cunninghamella elegans; Pine wilt disease; Pine tree; Phenolics